Structural organization of complexes of transfer RNAs with aminoacyl transfer RNA synthetases

Differences between mitochondrial and cytoplasmic transfer RNA and aminoacyl transfer RNA synthetases from rat liver.

The mitochondria of yeast,1 Neurospora,2 and Tetrahymena3 have been shown to contain transfer RNA (tRNA). Barnett et al.4 have found, in addition, that Neurospora mitochondria contain 15 aminoacyl-tRNA synthetases, at least three of which acylate only mitochondrial tRNA. The purpose of this communication is twofold. First we will present evidence showing that, as with eukaryotic microbial syste...

More evidence for a particle character of aminoacyl-transfer RNA synthetases isolated from rat liver.

Multienzyme complexes of eukaryotic aminoacyl-tRNA synthetases.

Eukaryotic aminoacyl-tRNA synthetases, unlike their prokaryotic counterparts, may occur as high-Mr multienzyme complexes. Recently, successful purification of synthetase complexes makes possible the elucidation of the structural organization of these high-Mr complexes. Although their physiological significance remains unknown, recent studies suggest some possible functional roles for these comp...

Affinity chromatography of aminoacyl-transfer ribonucleic acid synthetases. Cognate transfer ribonucleic acid as a ligand.

The use of tRNA affinity columns for the purification of aminoacyl-tRNA synthetases was investigated. A purification method for valyl-tRNA synthetase from Bacillus stearothermophilus is described that uses two affinity columns, one containing the pure cognate tRNA, and the other containing all tRNA species except the cognate tRNA. A method for the rapid preparation of the two columns was develo...

Mitochondrial-specific aminoacyl-RNA synthetases.

Numerous lines of evidencel'indicate that protein synthesis occurs within mitochondria, and tRNA8, 15, 16 and aminoacyl-RNA synthetases8 have been prepared from these organelles. More recently, two very interesting aspects of mitochondrial protein synthesis have been described: (a) Although amino acid incorporation by yeast cytoplasmic ribosomal systems is not inhibited by chloramphenicol,'7 pr...